Involvement of IL10 and granulocyte colonystimulating factor in the fate of monocytes controlled by galectin1

Abstract

The process of differentiation from monocytes to dendritic cells is critical in immune modulation. Monocyte apoptosis is a key regulator in balancing the immune response. Galectin1 has been reported to induce tolerogenic dendritic cells by the autocrine interleukin (IL)10 in monocytes. However, IL10 has been found to induce apoptosis in IL4/granulocyte macrophage colonystimulating factor (CSF) stimulating and nonstimulating monocytes, whereas galectin1 has not. After analyzing the factors secreted by galectin-1-activated CD14 monocytes isolated from the peripheral blood, the present study revealed that galectin1 upregulates IL10 and granulocyte (G)-CSF expression. Furthermore, GCSF inhibited IL10induced apoptosis, implying that galectin1 may enhance the immunemodulating functions of GCSF by inducing tolerogenic dendritic cells and maintaining their survival. Therefore, GCSF may be further applied in immune therapy, particularly in the IL10presenting microenvironment.