EVALUATION OF ANTIDIABETIC ACTIVITY OF ZANTHOXYLUM OVALIFOLIUM LEAF EXTRACTS

Abstract

Objective: The present study was aimed to investigate the antidiabetic activity of ethanolic and aqueous extract of Zanthoxylum ovalifolium on alloxan induced diabetic rat model in rats. Methods: The leaves of Zanthoxylum ovalifolium were evaluated for antidiabetic activity by using alloxan induced diabetic model in diabetic rats. Diabetes was induced by single intraperitoneal injection of alloxan (100 mg/kg) and rats were treated orally with test extracts, standard drug (glibenclamide 5 mg/kg) and vehicle for 21 d. The hypoglycemic effects and lipid profile of diabetic rats were assessed using diagnostic kits. Finally, histopathological studies were carried out for pancreas. Results: The acute toxicity studies revealed at the dose of 2000 mg/kg (b. w) of Zanthoxylum ovalifolium for ethanol and aqueous extract were found to be safe. A significant reduction (p<0.001) in blood glucose was observed in diabetic rats treated with different doses of extracts compared to untreated diabetic rats. The drug possesses a good hyperlipidemic effect by normalizing the lipid parameters. This was evidenced by histopathological studies; both glibenclamide and 400 mg/kg of Ethanolic extract does appear to be regulated diabetes at the cellular level, resulting in the restoration of near normal architecture pancreatic islet of langerhans. Conclusion: It can be concluded from our research findings that ethanolic and aqueous extract of Zanthoxylum ovalifoliumat high dose (400 mg/kg) exhibited significant antihyperglycemic activity than extract at low dose (200 mg/kg) in alloxan induced diabetic rats. These extracts also showed improvement in parameters like lipid profile as well as regeneration β-cells in the pancreas and so might be of value in diabetes treatment.