A novel sample pretreatment technique, headspace hanging drop liquid phase microextraction (HS-LPME) was studied and applied to the determination of flavors from solid clove buds by gas chromatography-mass spectrometry (GC-MS). Several parameters affecting on HS-LPME such as organic solvent drop volume, extraction time, extraction temperature and phase ratio were investigated. 1-Octanol was selected as the extracting solvent, drop size was fixed to 0.6 μL. 60 min extraction time at 25°C was chosen. HS-LPME has the good efficiency demonstrated by the higher partition equilibrium constant (K lh) values and concentration factor (CF) values. The limits of detection (LOD) were 1.5-3.2 ng. The amounts of eugenol, β-caryophyllene and eugenol acetate from the clove bud sample were 1.90 mg/g, 1.47 mg/g and 7.0 mg/g, respectively. This hanging drop based method is a simple, fast and easy sample enrichment technique using minimal solvent. HS-LPME is an alternative sample preparation method for the analysis of volatile aroma compounds by GC-MS.
CITATION STYLE
Jung, M. J., Shin, Y. J., Oh, S. Y., Kim, N. S., Kim, K., & Lee, D. S. (2006). Headspace hanging drop liquid phase microextraction and gas chromatography-mass spectrometry for the analysis of flavors from clove buds. Bulletin of the Korean Chemical Society, 27(2), 231–236. https://doi.org/10.5012/bkcs.2006.27.2.231
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