The Arabidopsis Athb-10 (GLABRA2) is an HD-Zip protein required for regulation of root hair development

293Citations
Citations of this article
107Readers
Mendeley users who have this article in their library.

Abstract

Homeodomain-leucine zipper (HD-Zip) proteins are putative transcription factors identified only in plants. Related Arabidopsis homeobox genes, isolated by virtue of sequence conservation within the helix-3 region of the homeodomain, fall into four families based on sequence similarity. This paper reports the characterization of Athb-10, a 747 amino acid protein belonging to the fourth HD-ZIP family. The studies indicate that, although less conserved, the leucine zipper of Athb-10 can functionally replace that of Athb-2 in an in vitro DNA-binding assay. Gene mapping experiments and sequence comparison analysis revealed that Athb-10 corresponds to GLABRA2, a homeodomain protein involved in trichome development. The mRNA expression analysis revealed that Athb-10/GLABRA2 is expressed not only in trichome-bearing organs, but also in the root. The analysis of wild-type and mutant plants showed that the Athb-10/GLABRA2 gene expression in the aerial part of the plant and in the root is affected by mutations at the TTG locus. Morphological analysis of the gl2-1 mutant revealed that the gene is necessary not only for local outgrowth of the trichome, but also for the regulation of root hair development in a subset of epidermal cells. Interestingly, the development of root hair cells in a position normally occupied by non-hair cells is dependent upon the ethylene regime in which the gl2-1 plants are grown. Sequence analysis of the gl2-1 allele revealed that the mutant gene encodes a truncated protein that might still retain a partial activity responsible for the formation of aborted trichomes and for the ethylene-dependent regulation of root hair formation.

References Powered by Scopus

Rapid production of full-length cDNAs from rare transcripts: Amplification using a single gene-specific oligonucleotide primer

4738Citations
N/AReaders
Get full text

Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension

2781Citations
N/AReaders
Get full text

Exploiting the triple response of Arabidopsis to identify ethylene-related mutants

1048Citations
N/AReaders
Get full text

Cited by Powered by Scopus

The TRANSPARENT TESTA GLABRA1 locus, which regulates trichome differentiation and anthocyanin biosynthesis in arabidopsis, encodes a WD40 repeat protein

818Citations
N/AReaders
Get full text

Transparent Testa Glabra2, a trichome and seed coat development gene of arabidopsis, encodes a WRKY transcription factor

677Citations
N/AReaders
Get full text

The late flowering phenotype of fwa mutants is caused by gain-of-function epigenetic alleles of a homeodomain gene

506Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Di Cristina, M., Sessa, G., Dolan, L., Linstead, P., Baima, S., Ruberti, I., & Morelli, G. (1996). The Arabidopsis Athb-10 (GLABRA2) is an HD-Zip protein required for regulation of root hair development. Plant Journal, 10(3), 393–402. https://doi.org/10.1046/j.1365-313X.1996.10030393.x

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 41

52%

Researcher 20

25%

Professor / Associate Prof. 17

22%

Lecturer / Post doc 1

1%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 55

70%

Biochemistry, Genetics and Molecular Bi... 21

27%

Social Sciences 2

3%

Chemical Engineering 1

1%

Save time finding and organizing research with Mendeley

Sign up for free