Transgenic potato tubers as a source of phenolic compounds. Localization of anthocyanins in the peridermis

Abstract

The localization of phenolic compounds in tubers of transgenic potatoes overexpressing enzymes of the flavonoid synthesis pathway, i.e. chalcone synthase (CHS), chalcone isomerase (CHI) and dihydroflavonol dehydrogenase (DFR), was examined by fluorescence microscopy. The study revealed the presence of flavonoids (anthocyanins) in cell vacuoles, mainly in the peridermis. A relatively higher concentration of anthocyanin compounds was observed in the tissue of transgenic potato tubers, revealing the expression of chalcone synthase (CHS). The increased content of flavonoids in transgenic potato tubers may affect their agrotechnical traits and may increase the nutritive value of the tubers.