Bioactive component analysis of seed coat hexane extract of Ardahan (Türkiye) walnut

Abstract

The walnut seed coat (WSC), a significant waste product that is underappreciated compared to other walnut parts, is responsible for the emergence of biological potential, has a favorable and protective effect on health, and includes a wealth of bioactive phytochemicals. Considering this situation, the aim of the study was to clarify the bioactive components in the WSC hexane extract. A hexane WSC extract (H-WSC) was initially produced to conduct the analysis. Four chemical components in the walnut seed coat were identified using the gas chromatography-mass spectrometry (GC-MS) method. Accordingly, the main components in hexane extracts are β-sitosterol (%32.91) and ethyl iso-allocolate (%52.06); other components are 3-(octadecyloxy)propyl (9E)-9-octadecenoate (%8.41) and santa camphor (%4.45).