Exploring endosymbionts and pathogens in Rhipicephalus sanguineus and Ctenocephalides felis felis with Oxford Nanopore Technology

Abstract

Fleas and ticks play a crucial role in public health as vectors of multiple diseases affecting humans and animals. Several rickettsial pathogens and endosymbionts are transmitted by fleas and ticks. Therefore, understanding this group of microorganisms is essential for fully grasping the spectrum of pathogens transmitted by vectors and the interactions between endosymbiotic microorganisms and their hosts. This study evaluated the presence and diversity of Rickettsiales species in fleas and ticks collected from the Santander department in Colombia. For the methodology a 16S gene amplification approach through Oxford Nanopore sequencing technologies in Rhipicephalus sanguineus and Ctenocephalides felis felis was used. Our findings revealed the presence of multiple pathogenic and endosymbiotic microorganisms, particularly from the Rickettsia and Wolbachia groups. We observed a clear association between Rickettsia species and ticks, while Wolbachia was predominantly found in fleas. Additionally, other important microorganisms were identified, including Anaplasma phagocytophilum, Rickettsia conorii, and different strains of Wolbachia that serve as endosymbionts in various arthropods. These results underscore the importance of fleas and ticks in the transmission of both pathogenic and endosymbiotic microorganisms. The distinct patterns of association between specific pathogens and vectors provide insight into their transmission dynamics. Identifying pathogens such as Anaplasma phagocytophilum and Rickettsia conorii further highlights the need for continued research into vector-borne diseases in Colombia. Understanding the interactions between endosymbionts and pathogenic microorganisms in these vectors could lead to the development of more effective strategies for controlling diseases transmitted by fleas and ticks.