Respuesta dieléctrica y bioquímica de un recubrimiento PLA-PGA-HAp-Quitosano-Colágeno sobre Ti6Al4V

Abstract

The interaction of a polymeric biomaterial witha culture medium and osteoblast cells, electrodeposited on Ti6Al4V was evaluated. The compound is integrated of polylactic acid-polyglycolic acid-hydroxyapatite, modified withcollagen and chitosan. The relative permittivity data embodied in a dielectric impedance spectrum identified the alpha and beta dispersions related to the ion exchange and the polarization of the cell membrane was calculated. Adhesion and cell proliferation was analyzed by epifluorescence microscopy, where it was observed on the third day of the cell culture process represented by mitosis core condensation and separation of the chromosomes. The surface morphology by SEM (Scanning Electron Microscope) and AFM (Atomic Force Microscope) biomaterial was observed and cellular activity was assessed by measuring alkaline phosphatase. Finally the best surface for adhesion and cell growthwas found by statistical analysis, which corresponded to the coating withthe highest concentration of chitosan and collagen.