Detection of Biofilm Production in Candida Species from the Vagina by Two Different Methods

Abstract

Aim: The incidence of fungal infections has increased today, and antifungal resistance has increased in such infections. It is known that most infections produced by Candida species are associated with biofilm formation. Materials and Methods: In this study, 192 patients diagnosed with yeast cytologically in cervico-vaginal smears between September 2015 and August 2020 were investigated. Results: When all Candida species studied with Congo Red Agar were evaluated, biofilm positivity was found to be 61.4% in non-albicans Candida species and 38.6% in C. albicans. Biofilm positive non-albicans Candida species were identified as 11 (15.7%) C. glabrata, 11 (15.7%) C. tropicalis, 6 (8.6%), C. guillermondii and 6 (8.6%) C. krusei. Conclusion: In fungal infections, the biofilm produced by the agent is directly proportional to antifungal resistance and invasion of the infection. Therefore, determining the biofilm production of the causative fungus is important in planning the treatment.