Transposition of Tn1213 encoding the PER-1 extended-spectrum -lactamase

Abstract

PER-1 is an extended-spectrum -lactamase that is encoded by a gene located in composite transposon Tn1213 made by two distinct insertion sequences, namely, ISPa12 and ISPa13. In vitro mobilization performed in Escherichia coli shows that Tn1213 is functional and is able to mobilize the blaPER-1 gene, although at a very low frequency (ca. 1 109).