Shoot Regeneration from Leaf Explants of American and Chinese Elm

Abstract

Shoots have been regenerated from greenhouse-grown Ulmus americana L. leaf sections on a modified Murashige and Skoog medium containing 0.1 μ m TDZ. After 2 months in culture, 73% ± 4% of explants treated with 0.1 μ m TDZ produced 5.5 ± 3.5 shoots per explant. There was no variation in regeneration potential detected among the seedlings tested. In addition, shoots were produced from > 80% of leaf explants from Ulmus parvifolia Jacq. Chemical name used: N-phenyl-N′-l,2,3-thiadiazole-5′yl urea (TDZ).