Generation of a zinc finger protein ZPR1 mutant that constitutively interacted with translation elongation factor 1α

Abstract

Zinc finger protein ZPR1 (ZPR1) binds to eukaryotic translation elongation factor 1α (eEF1α) in response to growth stimuli, and is also involved in transcription and cell cycle regulation. In this study, we characterized the interaction of ZPR1 and eEF1α and generated a ZPR1 mutant that constitutively interacted with eEF1α. ZPR1-ΔA (Δ193-246) bound to eEF1α independently of Zn2+ in vivo. This study indicates that ZPR1-ΔA (A193-246) is a useful tool to provide structural insights into ZPR1 and to investigate the biological significance of the interaction between ZPR1 and eEF1α.