Validation of a self-excising marker in the human pathogen aspergillus fumigatus by employing the βrec/six site-specific recombination system

Abstract

Recyclable markers based on site-specific recombination allow repetitive gene targeting in filamentous fungi. Here we describe for the first time functionality of the bacterial recombination system employing ß serine recombinase acting on six recognition sequences (β-rec/six) in a fungal host, the human pathogen Aspergillus fumigatus, and its use in establishing a self-excising resistance marker cassette for serial gene replacement. Copyright © 2020, American Society tor Microbiology. All Rights Reserved.