Analysis of carotenoid isomerase activity in a prototypical carotenoid cleavage enzyme, apocarotenoid oxygenase (ACO)

Abstract

Background: Isomerase activity for Synechocystis apocarotenoid oxygenase (ACO) has not been verified biochemically. Results: ACO exclusively produces all-trans-retinal from all-trans-apo-8β-cells-carotenol and is inhibited by linear polyoxyethylene detergents. Conclusion: ACO cleaves but does not isomerize all-trans-8β-cells-apocarotenol. Significance: This study clarifies the activity of and influence of detergent on ACO, a classical non-isomerizing member of the carotenoid cleavage enzyme family. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.