In vitro nematicidal activity of Juglone against Meloidogyne incognita race 2 infesting pomegranate

Abstract

Introduction. Juglans regia L. (walnut) is also known as the medicinal plant and native to the mountain ranges of central Asia. Recently, a severe infestation of root-knot nematodes (RKN, identified as Meloidogyne incognita race 2), was observed on Punica granatum L. (pomegranate). Aim. The present study aimed to investigate the effect of in-vitro nematicidal activity of the purified bioactive compounds (Juglone: 5-Hydroxy 1, 4-Napthoquinone) isolated from medicinal plant like walnut on Meloidogyne species associated with pomegranate under field conditions. Methods. Plants are cultivated at the agricultural farm in Solapur, (M.S.), India. Results. Roots of infected plants were heavily galled and soil samples collected from the affected plants second stage juveniles (J2). The ability of treatment schedule of Juglone was tested using an in-vitro method against RKN-Meloidogyne incognita race 2 infesting pomegranate. Juveniles in the control (distilled water; carbofuran) were compared with treated groups. The Juglone showed a 100% mortality in 5 μl/ 10 ml of distilled water/ 100 nematodes.