Effect of silver nanoparticles on the mortality pathogenicity and reproductivity of entomopathogenic nematodes

Abstract

Background and Objective: Scientists are concerning about using nanomaterials, such as silver nanoparticles (AgNP) in biological control of plant parasitic nematodes. In this study, it was evaluate that the side effect of AgNP on the non target nematodes, entomopathogenic nematodes (EPNs) that found naturally in the same soil environment and contribute to the insect pests control. Methodology: The effect of different concentrations (1500, 500, 200, 40 and 20 ppm) of silver nanoparticles on mortality of EPNs Heterorhabditis indica, Steinernema arenarium and Steinernema abbasi for (1, 2, 3, 4 and 5 days) was studied. And the effect on pathogenic properties of EPNs on Galleria mellonella was also studied. Results: It was found that, mortality percentage of EPNs depended on nano-Ag. Concentrations and the exposure time. There was a slight effect on pathogenicity, while there was a significant effect on EPNs reproductivity with the two concentrations (500 and 1500 ppm). Conclusion: Nanosilver can be used to control plant parasitic nematodes without excessive in order to don’t harm the useful nematodes, EPNs.