Anthelmintic activity of Mansoa alliacea against Pheretima posthuma: In vitro and in silico approach

Abstract

Objectives: Mansoa alliacea has been utilized to remedy many afflictions of humans. Literary works illustrate that it possesses numerous biological activities. Our analysis work aims to distinguish phyto-derived anthelmintic substances from M. alliacea against the enzyme β-tubulin and to consider the cause of its function in the molecular basis on Invitro and Insilico methods. Materials and Methods: In this study, Manosa alliacea was subjected to extraction using various solvents based on polarity and the extracts were analyzed by GC-MS. Then using Pheretima posthuma, in-vitro studies were done, and insilico studies have been conducted using PyRx tool. Subsequently, DruLiTo software was used to study drug-like predictions. Results: Tests showed that methanolic extract has the most important dose-dependent anthelmintic efficacy at various levels. By insilico studies, it shows that the four phytochemicals of M. alliacea are very likely against the β-tubulin. Utilizing contemporary strategies, these phyto-compounds from a natural origin might establish a reliable medication or support lead identification. Conclusion: Utilizing contemporary strategies, these phyto-compounds from a natural origin might establish a reliable medication or support lead identification. Identified hit compounds could be further taken for in vitro studies to examine their effectiveness versus helminths.