Transforming growth factor-β receptor and fibronectin expressions in aortic smooth muscle cells in diabetic rats

Abstract

Smooth muscle cells in arteries of diabetic rats and rabbits have unique properties including the overexpression of platelet-derived growth factor (PDGF) β-receptor compared with controls. Fibronectin, one of the increased components of extracellular matrices in diabetic arteries, plays an important role in the phenotypic change of smooth muscle cells from the contractile to the synthetic type with the expression of the PDGF β-receptor. Moreover, fibronectin synthesis is regulated by transforming growth factor-β (TGF-β). In this study, we report on the expression of TGF-β receptors in diabetic smooth muscle cells, by immunohistochemistry, cross-linking of 125I-TGF-β1 to cells and quantitative reverse transcription-polymerase chain reaction. We also report on the effects of TGF-β1 on fibronectin synthesis of diabetic smooth muscle cells by use of ELISA and immunoprecipitation, in order to clarify the role of TGF-β-fibronectin pathway in forming characteristic changes of diabetic smooth muscle cells. Cultured aortic smooth muscle cells of diabetic rats expressed TGF-β type II receptor about 8.7 times that of controls at the protein level and 5.7 times at the mRNA level, whereas the expression of the type I receptor did not differ between the two types of smooth muscle cells. These changes were accompanied by increased fibronectin synthesis in diabetic smooth muscle cells in response to TGF-β1. Furthermore, protein expression of fibronectin, and mRNA and protein of TGF-β type II receptor were increased in the diabetic aorta compared with the control aorta in vivo, implying the importance of the TGF-β-fibronectin pathway for the unique biology of smooth muscle cells in the diabetic artery.