ANTIMICROBIAL ANALYSIS OF SCHIFF BASE LIGANDS PYRAZOLE AND DIKETONE METAL COMPLEX AGAINST PATHOGENIC ORGANISMS.

Abstract

……………………………………………………………… Schiff base are flexi-dentate ligands, which are important to expand the Schiff base complexes. These complexes form excellent catalytic reaction by forming stable complex with metal ions and producing the biological activity against antibacterial, antifungal, anticancer and anti- malarial activities. In this study, we have explore the Minimum Inhibitory Concentration (both liquid & Disc method) of schiff base ligand such as Pyrazole, diketone and their metal (Ni, Cu, Co, Mn, Vn, Ca, Zn) bound complexes against various microbial pathogens. Minimum Inhibitory Concentration (MIC both liquid & disc method) were analyzed for the various human microbial pathogens such as Pseudomonas aeruginosa, Escherichia coli, Klebsilla pneumonia, Staphylococcus aureus. The sensitivity towards the various commercial antibiotics (Rifampicin, Streptomycin, Ampicillin, Gentamicin, Neomycin, Bacitracin, Erythromycin & penicillin) for the selected microbial human pathogens were also evaluated and compared. The MIC values indicate the most of the complexes have higher antimicrobial activity than the free ligand. The variation in the antimicrobial activity of different metal complexes against different microorganism depends on their impermeability of the cell or the differences in ribosomes in microbial cells. The lipid membrane surrounding the cell favours the passage of any lipid soluble materials and it is known that lipo solubility is an important factor controlling antimicrobial activity. The presence of low activity of some metal complexes is may be due to their low lipophilicity, because of which penetration of the complex through lipid membrane was decrease and neither block nor inhibit the growth of the microorganism.