Breast Cancer Screening in Low- and Middle-Income Countries: A Perspective From Malawi

Abstract

A new method for chemical protein modification is presented utilizing a dirhodium metallopeptide catalyst. The com- bination of peptide-based molecular recognition and a dirhodium catalyst with broad side-chain scope enables site-specific protein functionalization. The scope and utility of dirhodium-catalyzed biomolecule modification is expanded to allow reaction at physio- logical pH and in biologically relevant buffer solutions. Specific protein modification is possible directly in E. coli lysate, demonstrat- ing the remarkable activity and specificity of the designed metallo- peptide catalyst. Furthermore, a new biotin-diazo conjugate 1b is presented that allows affinity tagging of target proteins.