Effect of inoculum type and timing of application of Coniothyrium minitans on Sclerotinia sclerotiorum: Control of sclerotinia disease in glasshouse lettuce

Abstract

The effects of Coniothyrium minitans inoculum quality and an 8-week interval between inoculum application and crop planting on sclerotinia (Sclerotinia sclerotiorum) disease in three successive lettuce crops were investigated in a glasshouse trial. Spore suspensions of three isolates of C. minitans (Conio, IVT1 and Contans) applied at 108 CFU m-2 and a standard Conio maizemeal-perlite application (06 L m-2, 10 11 CFU m-2) were assessed for their ability to control S. sclerotiorum. Only the maizemeal-perlite inoculum (isolate Conio) consistently reduced sclerotinia disease. In the third lettuce crop only, isolates IVT1 and Contans formulated by Prophyta and isolate IVT as an oil-water formulation, all applied as spore suspensions, reduced disease at harvest compared with the untreated control. Recovery, viability and C. minitans infection of sclerotia buried during the 8-week period prior to each of the three lettuce crops, and of sclerotia formed on the crop, were tested. Only the maizemeal-perlite inoculum (isolate Conio) reduced the recovery of sclerotia buried in soil for weeks between inoculum application and crop planting, reducing their viability and increasing infection by C. minitans. Eight weeks was sufficient to enable C. minitans to infect sclerotia of S. sclerotiorum, and may account for disease control. After harvest of the second and third crops, maizemeal-perlite treatment (isolate Conio) reduced the number and viability of sclerotia recovered on the soil surface and increased infection by C. minitans compared with spore-suspension treatments. The effect of inoculum concentration and the influence of soil temperature (varying with time of year) on infection of sclerotia by C. minitans are discussed.