Synthesis of Extract-Bacterial Cellulose Composite Using Ageratum conyzoides L. and Chromolaena odorata L., Its Antibacterial Activities, and Biodegradability Properties

Abstract

Bacterial cellulose is a natural polymer produced by fermentation of coconut water using Acetobacter xylinum bacteria. This study aimed to synthesize a novel composite of bacterial cellulose impregnated with plant extracts that had an antibacterial activity that have the potential to be used as a food packaging material to maintain food quality. Pure bacterial cellulose (pure BC) was impregnated using Ageratum conyzoides L. leaf extract (AC-BC) and Chromolaena odorata L. leaf extract (CO-BC), which contain secondary metabolites with potential as antibacterial. The study began with the synthesis of pure BC, AC-BC, and CO-BC composites then characterized by SEM–EDX and FTIR, continued with antibacterial activity tests against S. aureus, S. typhimurium, E. coli, and their biodegradability tests. The results of SEM and FTIR characterization showed the success of the impregnation process for antibacterial compounds. The results of the antibacterial activity of AC-BC disc diffusion against S. typhimurium and E. coli showed good antibacterial activity of 9.82 mm and 8.41 mm, respectively. The similar result showed with the antibacterial activity of CO-BC disc diffusion against S. typhimurium and E. coli that showed good activity of 9.73 mm and 6.82 mm, respectively. On the other hand, the biodegradability test showed that the impregnation of bacterial cellulose slowed down the degradation process in the soil. This study confirmed the potential application of bacterial cellulose-plant extracts as an active and biodegradable food packaging.