Phytochemical Screening, Spectroscopic Examination and Antimicrobial Evaluation of PHE (Poly Herbal Extract) of Selected Indian Medicinal Herbs

Abstract

Methanolic extract of important medicinal herbal plants like Marsilea-quadrifolia (whole plant), Trigonella-foenum-graecum (whole plant), Leaves of Mangifera-indica, Seeds of Eugenia-jambolana, Flowers of Cassia-auriculata, Flower of Musa- paradisiaca, Leaves of Aegle-marmelos and equal ratio of the mixture of the seven plants (Poly Herbal Extract - PHE) was prepared. The phytochemical screening for all the individual plants and PHE have been studied by using UV, IR, TLC and AAS. The results obtained from spectroscopic analysis indicated the presence of alkaloid, carbohydrates, protein, phenols, tannin, saponin, triterpenoids, glycosides, phytosterols, gums and mucilage. The results of TLC for seven plants and PHE showed the Rf factor in confirmation with phytochemical and spectrometric analysis. The atomic absorption studies reflected the presence of copper, iron, magnesium and zinc metals in PHE. Antimicrobial studies for all the individual plants and PHE were carried towards gram positive bacteria Bacillus subtilis and gram negative bacteria Pseudomonas aeruginosa, Escherichia coli respectively. The in vitro antimicrobial studies also revealed that PHE showed better activity against E. coli which is comparable with the standard ciprofloxacin.