Common FXIII and fibrinogen Polymorphisms in abdominal aortic aneurysms

Abstract

Introduction: Abdominal aortic aneurysms (AAA) are characterized by a progressive dilatation of the abdominal aorta, and are associated with a high risk of rupture once the dilatation exceeds 55 mm in diameter. A large proportion of AAA develops an intraluminal thrombus, which contributes to hypoxia, inflammation and tissue degradation. We have previously shown that patients with AAA produce clots with altered structure which is more resistant to fibrinolysis. The aim of this study was to investigate genetic polymorphisms of FXIII and fibrinogen in AAA to identify how changes to these proteins may play a role in the development of AAA. Methods: Subjects of Western/European descent, ≥55 years of age (520 AAA patients and 449 controls) were genotyped for five polymorphisms (FXIII-A Val34Leu, FXIII-B His95Arg, FXIII-B Splice Variant (intron K nt29576C-G), Fib-A Thr312Ala and Fib- B Arg448Lys) by RT-PCR. Data were analysed by x2 test and CubeX. Results: The FXIII-B Arg95 allele associated with AAA (Relative risk - 1.240, CI 1.093-1.407, P = 0.006). There was no association between FXIII-A Val34Leu, FXIII-B Splice Variant, Fib-A Thr312Ala or Fib-B Arg448Lys and AAA. FXIII-B His95Arg and FXIII-B Splice variant (intron K nt29576C-G) were in negative linkage disequilibrium (D′ = 20.609, p = 0.011). Discussion: The FXIII-B Arg95 variant is associated with an increased risk of AAA. These data suggest a possible role for FXIII in AAA pathogenesis. RASA and DJAS were supported by The Garfield Weston trust into diseases of the Heart. KIB was supported by The British Heart Foundation Clinical Research Training Fellowship, FS/12/26/29395. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.