Concentrações de Eugenol para anestesia profunda e toxidade aguda em juvenis de piavuçu ( Leporinus macrocephalus)

Abstract

Three works were undertaken to evaluate the anesthetic capacity of eugenol in “piavuçu” juveniles; the influence of different concentrations on the anaesthetic effect, the acute toxicity (LD 50) and the Time for Lethal Dose (TLD) for the species. Seventy-two fish (1.77 + 0.69 g) were submitted to eight different eugenol concentrations (25; 37.5; 50; 62.5; 75; 100; 125 and 150 mg L-1 were submitted to five concentrations (25; 37.5; 50; 62.5; 75 mg L-1 ); 150 fish ) for ten minutes; and 150 fish were submitted to 37.5 mg/L for five time intervals (300; 600; 900; 1200; 1500 seconds). Eugenol concentration had strong influence on the anesthetic effect for the species; LD for 600 seconds of exposure were LD01 25.21 mg L-1 LD99 65.05 mg L-1 , LD50 45.13 mg L-1 eugenol is recommended.