ISOLASI BAKTERIPENGHASIL ANTIBIOTIKA DARI CAIRAN KANTUNG TUMBUHAN KANTONG SEMAR (Nepenthes spp.) CAGAR ALAM LEMBAH HARAU SUMATERA BARAT

Abstract

Exploration of antibiotic-producing micro organisms continue to get good anti biotic-producing isolates. Many producers of anti biotic substances have been isolated, but a small portion of them are proven to be beneficial to both, especially in the health field. Some antibiotic-producing micro organisms found in places with diverse condition sone of which isa liquid Nepenthes spp. The fluidin the saccontaining plant Nepenthes chemical compounds, acidic and capable of digesting insects that go into the bag. This of course affects the fluid sacas a growing medium for bacteria. Some bacteria are capable of producing antibiotics to the specific need sof the bacteria to survive unfavorable conditions. Harau Valley Nature Reserveis one ofthe habitats where the growing Nepen the sin West Sumatra. However, no studies have discussed the presence of antibiotic-producing bacteria in the fluid sacin this area. Sampling sac fluid of Nepenthes spp., conducted by purposive sampling method. Antibiotic testing of bacterial isolates was conducted using paper disc. From the results of bacterial isolation was obtained 137 bacterial isolates were scattered in four different type sof fluid sac Nepenthes including N. ampullaria, N.gracilis, N.eustacya and N. albomarginata. Antibiotic testing ten isolates were isolated from the fluid sac Nepenthes spp., generally indicates the ability of antibiotic stoutest the bacteria E.coli and S. aureus. Isolates KS.4-3 havethe best antibiotic against E.coli with inhibition zone diameter of 29.5 mm and isolatees reached KS. 1-7 against the bacteria S.aureus with in hibition zone diameter of 26.3mm reach.