Anticaries and α-amylase inhibitory activity of jasminum arborescens roxb. (oleaceae) leaves extract

Abstract

The aim of the present study was to determine anti caries and a-amylase inhibitory activity of leaf extract of Jasminum arborescens Roxb. (Oleaceae). Anti caries activity was determined by Agar well diffusion assay against seven clinical isolates of Streptococcus mutans (Sm-01 to Sm-07) recovered from dental caries subjects. Enzyme inhibitory activity was tested against a-amylase by spectrophometric method using starch as substrate. The extract exhibited dose dependent inhibition against cariogenic isolates. Among seven isolates, isolate Sm-04 and Sm-06 were inhibited to higher and least extent respectively. The extract was found to cause inhibition of a-amylase activity in a dose dependent manner and its IC50 value was found to be 17.45 mg/ml. The inhibitory activity could be attributed to the presence of secondary metabolites. The plant may be a potential source for development of agents which are active against dental caries pathogens and for diabetes mellitus.