Developmental control of xylem hydraulic resistances and vulnerability to embolism in Fraxinus excelsior L.: Impacts on water relations

Abstract

The hydraulic properties and leaf gas exchanges of Fraxinus excelsior L. branches differing by their age and their vertical crown position, but in comparable ambient air conditions (vapour pressure deficit and global radiation) were compared. The variations in leaf let water potential ψ(leaflet), leaflet stomatal conductance and transpiration rate, E, were small between different branches of the same crown. Whole branch hydraulic resistances (r(branch)), and partitioning between leaf (r(leaf)) and xylem resistance (r(xylem)) were assessed with a high pressure flowmeter. r(leaf) represented 90% and 10% of r(branch) for upper and lower crown branches, respectively. The changes resulted from increases in r(xylem) caused by the formation of short shoot internodes mostly located in secondary axes. However, leaf area- specific branch resistances (r(branch)/*=r(branch) x LA) were nearly constant throughout the crown. This was consistent with the vertical variations in ψ(leaflet) because r(branch)/* x E represents the water potential drop from the trunk to the leaves. Because r(xylem) was higher, lower ψ(xylem) values were predicted in lower crown rachises. However, rachises from lower crown branches were less vulnerable to embolism than in upper branches (ψ(xylem) at onset of embolism, ψ(cav), were -3 and -2 MPa, respectively). It was concluded that r(xylem) increased with branch age, but r(branch)/* remained constant because LA decreased. As a consequence, E was maximized and ψ(xylem) remained above ψ(cav). This suggested that, in Fraxinus, leaf gas exchanges and leaf areas were coupled with xylem hydraulic capacities probably through a control of bud activity.