DEVELOPMENT AND VALIDATION OF UV SPECTROSCOPY FOR THE ESTIMATION OF RUTIN FROM ETHANOLIC EXTRACT OF COCCULUS HIRSUTUS LEAVES

Abstract

Rutin is a citrus flavonoid glycoside and exhibits anti-inflammatory, antihepatotoxic, antiulcer, antiallergic, antiviral and antioxidant activity. It is found in many herbal plants. The aim of this research work was to develop and validate a simple, accurate, precise and economic UV spectroscopic method for the determination of rutin in Cocculus hirsutus. The estimation of rutin by spectroscopic with maximum absorption at lambdamax 262.10 nm using developed solvent [n-butanol : water : acetic acid (7:1:1)]. Beer-Lambert's law was obeyed in the concentration range of 0-18 mug/ml and was described by the regression equation y = 0.032x + 0.008 with a regression coefficient (r2) = 0.999 (n = 5). For Rutin, the value of molar absorptivity and Sandell's sensitivity were 1.6429 x 106 L/mol/cm and 0.0372 x 10-2 mug/cm2, respectively and of LOD and LOQ were found to be 0.6135 and 1.8593 mug/ml, respectively. The percentage recovery of rutin was found to be 98.22 %. The % RSD for intraday and interday precision was 1.33 and 1.52 respectively. It showed excellent % RSD which is less than 2. The developed method was validated in terms accuracy, precision, linearity and robustness. The statistically validated results indicate that the proposed method has good sensitivity, accuracy and precision. The method is simple and economic as compare to chromatographic methods. The developed method was successfully applied for the determination of Rutin in herbal plants and its product.