Pyrite-assisted denitrification in recirculated biofilter tolerates pH lower than 5

Abstract

This study demonstrates the feasibility of pyrite-assisted denitrification to treat mildly acidic wastewaters and proposes a convenient solution for nitrogen removal from acidic mining waters. Pyrite is a highly available and low-cost electron donor for autotrophic denitrification, whereas mining waters represent a source of nitrogen (N) contamination due to the use of N-containing explosives during mining operations and cyanide (CN)-based compounds for ore processing. In this study, the denitrification performance of a recirculated pyrite-packed biofilter (RPPB) treating simulated N-contaminated acidic mining waters is evaluated under decreasing pH conditions. The effect of feed organic carbon on the autotrophic process has been also investigated. N removal efficiencies (NRE) > 60% could be achieved at feed pH ≥ 4.5 and HRT ≥ 5 h. Decrease of influent pH to 3.0 reduced the NRE to 20%. The addition of 10 and 20 mg/L of ethanol to the influent did not significantly impact NRE of the RPPB. Practitioner Points: Pyrite-assisted denitrification is proposed for treating acidic wastewaters. Nitrogen removal >60% was maintained at feed pH as low as 4.7. Decrease of feed pH to 3 strongly inhibited denitrification. The presence of organic carbon in the feed did not affect the autotrophic process.