Isolation of active compounds from the leaf extract of patah kemudi (Abroma augusta L.) and its anti-inflammatory activity

Abstract

Patah kemudi (Abroma augusta L.) is one of the shrubs that live on the edge of a river. A. augusta contains several secondary metabolite compounds including alkaloids, triterpenoids, steroids and flavonoids. The aim of this study was to isolate the compounds contained in the leaves of Abroma augusta and determine anti-inflammatory activity. Extraction and fractionation were performed by multilevel maceration. Isolation of compounds was carried out by column chromatography. The anti-inflammatory activity test was done using the Winter method. The n-hexane extract was separated by vacuum column chromatography and continued with gravity column chromatography to obtain isolate 8.1, which was then characterized using UV-Vis and IR spectrophotometer. The results of UV-Vis spectrophotometer on isolate 8.1 showed absorption at a maximum wavelength of 268 indicating a conjugate double bond which may be an aromatic originating from steroid compounds. While, the results of IR spectrophotometer characterization from isolate 8.1 manifested the presence of steroid compounds. The results of the activity test of isolate 8.1 with a dose of 10 mg presented the best anti-inflammatory activity compared to the positive control of diclofenac sodium. From the results of the GCMS shows that isolates contain γ-sitosterol compounds with a value of m/z 414.