PNA Hybeacons for analysis of human mutations related to statin-induced myopathy

Abstract

Aminoalkyl and alkyne-tagged PNA Hybeacons have been synthesized, labeled with fluoresce in via conventional amide bond or triazole formation (click chemistry) and used to detect single nucleotide polymorphisms (SNPs) implicated in statin-induced myopathy. The PNA hyBeacons gave much better mismatch/mutant discrimination than conventional DNA hyBeacons but smaller fluorescence changes on melting.