Differential expression of two pro-opiomelanocortin mRNAs during temperature stress in common carp (Cyprinus carpio L.)

Abstract

Pro-opiomelanocortin (POMC) is the precursor of a number of biologically active peptides, including adrenocorticotropic hormone, α-melanocyte- stimulating hormone and β-endorphin, which are released by the pituitary glands of fish as well as mammals. To quantify the levels of expression of the two POMC mRNAs relative to one another during the response of the common carp to temperature-induced stress, we used reverse transcriptase PCR combined with capillary electrophoresis and laser-induced fluorescence detection. The ratio of POMC-I mRNA to POMC-II mRNA determined in wild-type and four isogenic carp strains was found to be strain-dependent and influenced by temperature. In strain E20 x R8, the ratio had altered in favour of POMC-I from 1:3.2 (POMC-I:POMC-II) in fish adapted to 24 °C to 1:1.2 in fish adapted to a decrease of 9 °C in ambient temperature. A rapid drop in temperature from 24 to 15 °C decreased the POMC mRNA ratio at the expense of POMC-I from 1:1.9 in the control fish (strain E4 x R3R8) to 1:4-2 3 h after the temperature drop of 9 °C. We conclude teat both POMC genes are expressed in the common carp and that their expression ratio is strain- dependent and changes in response to ambient temperature.