Kinetic spectrophotometric methods for the determination of isatin

Abstract

Simple, rapid and sensitive kinetic spectrophotometric methods are described for the analysis of isatin in pure form and in mixture. The procedures were based on the kinetic investigations and studies were done upon the oxidation of isatin with potassium permanganate in basic medium. Various parameters affecting the colour developments were investigated and parameters were optimized. The reliability and the analytical performance of the proposed methods were validated for its linearity, range, accuracy and precision. The calibration curves were linear over the range of 5-50 μg mL-1. The limit of detection and limit of quantitation of the two methods ranges from 3.4 × 10-4-0.878 and 1.05 × 10-3-2.663, respectively. The possible mechanism of the reaction is also proposed.