Exploring SNPs (Single Nucleotide Polymorphisms) of Myostatin gene in coding region in Bali cattle

Abstract

The gene MSTN is a member of the superfamily growth gene transforming growth factor β (TGF-β) that functions to suppress muscle growth. The purpose of this study was to analyze the polymorphisms of MSTN genes in the coding region (exon 1, 2, and 3) in Bali cattle kept at the Breeding Center for Bali cattle (BPTU-HPT) in Denpasar. The number of samples used was 52 heads male of Bali cattle aged 2-3 years. SNP identification was done by amplifying the coding region, exon 1, exon 2 and exon 3 MSTN genes using direct sequencing method. Analysis of MSTN gene sequences in Bali cattle was carried out by using the BioEdit and MEGA7 programs. The present study found a total of 18 SNP mutations namely point mutation that are 4 SNP (g.313 C>A, g.324 T>A, g.330 T>G, g.400 G>A) in exon 1, 1 SNP (g.2609 G>A) in exon 2, and 13 SNP (g.4821 C>A, g.4838 C>T, g.4842 A>C, g.4849 T>C, g.4868 C>A, g.4873 C>G, g.4900 T>G, 4905 C>G, g.4957 C>G, g.4959 C>A, g.4969 T>A, g.4974 T>C, g.5044 C>A) in exon 3. SNPs found in the MSTN gene in Bali cattle might be used as candidates for Marker Assisted Selection, especially in Bali cattle.