Crystallization and preliminary crystallographic analysis of endo-1,3-β-glucanase from alkaliphilic Nocardiopsis sp. strain F96

Abstract

Endo-1,3-β-glucanase, an enzyme that hydrolyzes the 1,3-β-glycosyl linkages of β-glucan, belongs to the family 16 glycosyl hydrolases, which are widely distributed among bacteria, fungi and higher plants. Crystals of a family 16 endo-1,3-β-glucanase from the alkaliphilic Nocardiopsis sp. strain F96 were obtained by the hanging-drop vapour-diffusion method. The crystals belonged to space group P21, with unit-cell parameters a = 34.59, b = 71.84, c = 39.67 Å, β = 90.21°, and contained one molecule per asymmetric unit. The Matthews coefficient (VM) and solvent content were 1.8 Å3 Da-1 and 31.8%, respectively. Diffraction data were collected to a resolution of 1.3 Å and gave a data set with an overall Rmerge of 6.4% and a completeness of 99.3%. © 2006 International Union of Crystallography. All rights reserved.