Single‐cell RNA sequencing reveals a mechanism underlying the susceptibility of the left atrial appendage to intracardiac thrombogenesis during atrial fibrillation

Abstract

Graphical Abstract • Single-cell RNA-sequencing revealed atrial appendage-specific transcriptional heterogeneity of atrial fibrillation patients. • Differences in the local expression of anticoagulants by endocardial endothe-lial cells (EECs) contribute to the left atrial appendage (LAA)-specific propensity for thrombogenesis. • Deficiency of the anticoagulants TFPI and TFPI2 in the EECs of the LAA can be attributed to the proteolytic activity of ADAMTS1 in response to shear stress. Clin. Transl. Med. 2023;13:e1297. wileyonlinelibrary.com/journal/ctm2 https://doi. Abstract Background: Atrial fibrillation (AF) is associated with an increased risk of thrombosis of the left atrial appendage (LAA). However, the molecular