Construction of a pair of practical Nocardia-Escherichia coli shuttle vectors

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Abstract

We constructed a pair of Nocardia-Escherichia coli shuttle vectors, pNV18 and pNV19, by combining the mycobacterial plasmid pAL5000 with the E. coli vector pK18 or pK19. These vectors have a number of useful features, including small size (4.4 kb), a multiple cloning site, and blue/white selection. To our knowledge, pNV18 and pNV19 are the first cloning vectors for practical use in Nocardia spp.

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Chiba, K., Hoshino, Y., Ishino, K., Kogure, T., Mikami, Y., Uehara, Y., & Ishikawa, J. (2007). Construction of a pair of practical Nocardia-Escherichia coli shuttle vectors. Japanese Journal of Infectious Diseases, 60(1), 45–47. https://doi.org/10.7883/yoken.jjid.2007.45

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