Effects of different types of biochar on basic properties and bacterial communities of black soil

Abstract

In order to explore the effects of biomass carbon on the basic properties of black soil and bacterial diversity, in this study, the combination of indoor culture and high-throughput sequencing was used to analyze the nutrient changes and bacterial communities of black soil after adding biochar. The results showed that compared with CK, soil pH, organic matter and available potassium increased significantly after adding biochar (P < 0.05), and catalase, invertase, urease, and phosphatase increased substantially, while polyphenol oxidase decreased. A total of 17 phyla, 51 known genera were detected by high-throughput sequencing, among which Proteobacteria was the dominant phyla. The dominant genera including Sphingomonas, Mizugakiibacter, Bacillus, Marmoricola, Rhodanobacter, and Streptomyces. From the analysis of bacterial diversity, it can be concluded that the bacteria ace, chao1, Shannon and Simpson index of black soil increased after adding biochar, compared with CK in redundancy analysis (RDA), AN, NO3--N, NH4+-N had a greater impact on microbial communities. The microbial functional bacteria AOA and cbbl were significantly correlated with pH, SOM, AN, NH4+-N, and NO3--N (p < 0.05). Therefore, the addition of biochar can not only improve soil nutrients but also plays an important role in maintaining the diversity of soil bacterial communities.