Determination of multi-class mycotoxins in tartary buckwheat by ultra-fast liquid chromatography coupled with triple quadrupole mass spectrometry

Abstract

Considering crops are susceptible to toxicogenic fungi during plantation, pre-processing and storage, an ultra-fast liquid chromatography coupled with triple quadrupole mass spectrometry (UFLC-QTrap-MS/MS) method was developed and validated for simultaneous determination of the 12 most frequent mycotoxins, including aflatoxin B1, B2, G1, G2, HT-2, T-2 toxin, ochratoxin A, fumonisin B1, B2, zearalanone, zearalenone, and deoxynivalenol, in 14 batches of Tartary buckwheat cultivar, collected from different origins in Sichuan Province, China. Differing from those complicated approaches, a simple and cost-efficient pretreatment method based on dilute-and-shoot was employed. Based on optimized chromatographic and mass spectrometry conditions, these 12 mycotoxins could be analyzed with high correlation coefficients (all over 0.995), high precision (RSD 0.47–9.26%), stability (RSD 0.72–11.36%), and recovery (79.52% to 108.92%, RSD 4.35–14.27%). Furthermore, this analysis method exhibited good determination performance with little disturbance of the matrix effect. Finally, this proposed method was applied for 14 batches of Tartary buckwheat seeds, in which aflatoxin B1 (AFB1) was detected in one moldy cultivar, Meigu No. 2, with its concentration exceeding the maximum residue limits set by EU regulations. The method thus established, which has significant advantages, could provide a preferred determination approach candidate for measurement of multiple mycotoxins measurement in Tartary buckwheat, even other kinds of foodstuffs.