Effect of alterations in cuticular wax biosynthesis on the physicochemical properties and topography of maize leaf surfaces

Abstract

The leaf surface properties of 11 cuticular wax mutants of maize were Characterized, and this information was used to identify the quantitative relations among distinct leaf surface traits. Compared with the wild-type maize, these mutants were reduced 3-24% in their leaf surface hydrophobicity, 20-88% in the mass of cuticular waxes on their leaves, and 52-94% in the percentage of planar leaf surface area covered with epicuticular crystalline waxes. They also differed in the presence and abundance of the epicuticular crystalline waxes in each of seven structural classes. With the exception of one mutant, the mass of cuticular waxes produced by these mutants was positively correlated with the number of epicuticular crystalline waxes per unit area on their leaves. Furthermore, an increase of 0.4 mg of cuticular wax per gram of leaf (dry weight) was associated with a 1% increase in leaf surface area covered by epicuticular crystalline waxes, and this 1% increase was associated with a 2° increase in the contact angle of a water droplet on the leaf surface. Linear differences in the leaf surface hydrophobicity were associated with exponential differences in the mass of the cuticular waxes produced. Quantitative knowledge of these leaf surface properties is highly relevant to the interactions of leaves with environmental factors such as microbes, insects, agricultural chemicals, and pollutants.