Sida acuta leaf extract attenuates oxidants-induced animal model of nephrotoxicity and hepatotoxicity

Abstract

Curative potential of Sida acuta Burm. f. (Malvaceae) leaves on hepatotoxicity and nephrotoxicity induced by carbon tetrachloride (CCl4) and rifampicin was studied. This was aimed at providing a potent therapeutic alternative to conventional drugs in the management of liver and kidney diseases. Carbon tetrachloride (3 ml/kg bw) and rifampicn (250 mg/kg bw) were administered to induce liver and kidney damage in selected groups of albino rats. Graded doses of Sida acuta leaves extract as well as silymarin (200 mg/kg bw) were then post-administered to experimental animals placed into eight groups of five animals each. Biomarkers of oxidative stress such as lipid profile, alanine aminotransferase (ALT), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), superoxide dismutase (SOD), catalase (CAT), urea, uric acid, bilirubin and malonidialdehyde (MDA) were determined. Histopathological observations of both renal and hepatic tissues of experimental animals were also performed. Animals exposed to CCl4 and rifampicin without treatment exhibited significant derangement in lipid profile, elevated levels of ALT, ALT, ALP, urea, uric acid, bilirubin and MDA in the serum and tissues homogenates. Superoxide dismutase and catalase activities were significantly inhibited while level of GSH was depleted. Following treatment with S. acuta extract, all deranged parameters including histological alterations were restored in a dose-dependent manner comparable to animals treated with silymarin. Biochemical and histopathological data obtained from the present study confirmed that Sida acuta is a potential antioxidant plant that can be exploited in the management of liver and kidney dysfunctions.