Application of CRISPR/Cas9 in Wheat Genetic Improvement

Abstract

This study discusses the application of CRISPR/Cas9 technology in wheat genetic improvement, highlighting its potential in overcoming traditional breeding limitations. The study covers the working principles of CRISPR/Cas9, its advantages in precise genome editing, and its utility in enhancing traits such as disease resistance, stress tolerance, yield, and nutritional content in wheat. Case studies illustrate successful implementations where susceptibility genes were edited to bolster disease resistance, and specific genes were targeted to improve stress tolerance and grain quality. Despite its potential, challenges such as low editing efficiency, off-target effects, and regulatory hurdles remain. Nonetheless, the integration of CRISPR/Cas9 with other methods shows promise for future wheat breeding, aiming to ensure food security amidst growing global demands.