Copper oxide nanoparticles impregnated antibacterial surgical gloves for potential application in prevention of nosocomial transmission infections during nursing

Abstract

This work showed the preparation of Copper oxide nanoparticles (CuO NPs) from leaf extract of Cinnamomum camphora (C.camphora) by a green synthetic method. To prepare CuO NPs, about 10 ml of 0.01 M copper sulphate and 30 ml of C.camphora extract were mixed by heating for 60 min at a temperature of 80 °C. Various techniques such as x-ray diffraction analysis (XRD), Energy-dispersive spectroscopic analysis (EDS), Fourier-transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), UV-vis spectroscopy (UV-vis) and Transmission electron microscopy (TEM) were used for the characterization of biosynthesized CuO NPs. The formation of CuO NPs was indicated by gradual color change of brownish yellow solution into dark brown. Poly-dispersive and spherically shaped NPs were seen from TEM images with an average particle size of about 23 nm. FTIR results confirmed that polyphenols were capped onto the surface of the formed CuO NPs. On the other hand, the Gloves coated with CuO NPs were extremely successful in suppressing contamination of the outside glove surface with nosocomial-resistant microorganisms and hence beneficial of their use in the food sector or clinical context. CuO NPs-coated latex gloves significantly reduced all experimental bacteria within 30 s, including Methicillin-resistant Staphylococcus aureus, Vancomycin resistant enterococci, Escherichia coli, Acinetobacter, and Candida albicans (P