Effects of Mulberry Leaves and Pennisetum Hybrid Mix-Silage on Fermentation Parameters and Bacterial Community

Abstract

The silage quality and bacterial community of hybrid Pennisetum (P. hydridum × P. amer-icanum) with or without 30% and 50% mulberry leaves for 3, 7, 14, and 30 days were investi-gated. Results showed that compared with the 100% hybrid Pennisetum group, more lactic acid (40.71 vs. 80.81 g/kg dry matter (DM)), acetic acid (10.99 vs. 31.84 g/kg DM), lactic acid bacteria (8.46 vs. 8.51 log10 cfu/g fresh matter), water-soluble carbohydrates (2.41 vs. 4.41 g/100 g DM), crude protein (4.97 vs. 10.84 g/100 g DM), and true protein (3.91 vs. 8.52 g/100 g DM) content as well as less neutral detergent fiber (67.30 vs. 47.26 g/100 g DM), acid detergent fiber (33.85 vs. 25.38 g/100 g DM), and yeast counts (4.78 vs. 2.39 log10 cfu/g fresh matter) and an appropriate pH (3.77 vs. 4.06) were found in silages added with 50% mulberry leaves at 30 days of ensiling. Moreover, the addition of mulberry leaves also influenced the relative abundance of the bacterial community. The relative abundance of Firmicutes increased and Proteobacteria decreased when mulberry leaves were added. Weissella and Lactobacillus abundance also increased. To sum up the above, mixing with 50% mulberry leaves yielded the greatest fermentation quality in this study. In conclusion, mixing with mulberry leaves could be a reasonable way to improve the quality of hybrid Pennisetum silage.