Mesenchymal Stem Cell-conditioned Medium Promote the Recovery of Skin Burn Wound

Abstract

Background: Mesenchymal stem cell-conditioned medium (MSC-CM) is the substance extracted from mesenchymal stem cell culture, contains a lot of potential cytokines and growth factors as regenerative agent. Objective: The aim of this study was to investigate the role of MSC-CM on the burn wound regeneration pattern of white rat (Rattus norvegicus). Methodology: Rat was anesthetized using combination of 10% ketamine and 2% xylazine at the dose rate of 75 and 5 mg kgG1 b.wt., respectively. The burn wound condition was created on the dorsal area of each rat. The burn wound area of control group was treated with Bioplacenton®, while MSC-CM group was topically treated with MSC-CM cream twice a day. The diameter of burn wound was measured every 5 days from wounded. Skin wound tissues were collected at 4 h, followed by 2, 5, 10, 15, 20, 25 and 30 days after wounded and then were processed by paraffin method. Tissue samples were visualized by using hematoxylin-eosin and Masson’s trichrome stain. The polymer-based immunohistochemistry method was employed to detect the presence of basic fibroblast growth factor (bFGF) as important growth factor in wound healing process. Result: The results showed that MSC-CM promote the recovery of skin burn wound in white rat, as indicated by: (1) Acceleration of wound closure, (2) Greater numbers of fibroblasts, (3) High density of collagen fibers and (4) Greater numbers of blood vessels in MSC-CM group compare with control group. Conclusion: Since the number of bFGF immunoreactive cells increased significantly during recovery proccess in MSC-CM group compare with control group, it was suggested that bFGF plays important role on the tissues regeneration of skin burn treated by MSC-CM