Polygalacturonase Production by Aspergillus Niger Solid State Fermentation on Barley Bran and Sugar Beet Pulp Mixture

Abstract

In this research, barley bran mixtures with sugar beet pulp were used for polygalacturonase enzyme production by Aspergillus niger fungus in solid state fermentation method for the first time. Effect of some factors including carbon sources relative composition, fermentation time, fermentation temperature, inoculation amount, and moisture content were investigated and optimized amounts were selected. Different mixtures of barley bran and sugar beet pulp with various relative compositions were studied and highest enzyme activity was found for 50% w/w of barley bran and sugar beet pulp. Incubation temperature of 30 °C, time duration of 7 days, moisture content of 80% w/w, and 10 million spores per gram of dry substrate were selected as optimum amounts.