Screening, Identification, Characterization and Production of Bacterial Lipase from Oil Spilled Soil

Abstract

Lipases are glycerol ester hydrolases that catalyze the hydrolysis of triglycerides to free fatty acids and glycerol. Bacterial Lipase producers were isolated from oil spilled soil from vegetable oil processing factories. One of the twenty isolated strain exhibited a greater zone of clearance than the others indicating higher lipase activity was selected and identified based on their morphological and physicochemical characteristics and 16s rRNA sequencing. The effect of incubation time, medium pH, temperature, agitation, inoculums concentration, carbon source and nitrogen source for the lipase production was studied. The lipase production was maximum at pH7, temperature 370C and incubation time 48 hours by the lipase producing bacteria BLP2 Pseudomonas gessardii. Increased enzymatic production was obtained when the organisms were cultured in medium supplemented with 1% protease peptone by Pseudomonas gessardii (168.7Uml-1). The results of the present study demonstrate that the Pseudomonas gessardii is ideal for extracellular lipase production at industrial level.