UJI AKTIVITAS EKSTRAK ETANOL 96% DAN FRAKSI DAUN KIRINYUH (Chromolaena odorata L.) TERHADAP PROPIONIBACTERIUM ACNES

Abstract

Chromolaena odorata L. leaves can be used as an antibacterial because of contain flavonoid compounds. The secondary metabolite content of Chromolaena odorata L leaves include flavonoids, tannins, saponins, and steroids which have antibacterial activity. The aim of this study was determine the antibacterial activity of  ethanolic 96% and fraction of Chromolaena odorata L leaves and determine the concentration of  Chromolaena odorata L fraction and ethanol 96% extract which was most effective as an antibacterial against Propionibacterium acnes. The method was used to determine the MIC was a solid dilusion method, while the determination of inhibitory zone by the paper disk diffusion method at concentrations of 10%, 15% and 20% in each sample. The MIC showed hexane fraction is 15%, ethylacetate fraction is 15% and water fraction is 10%. The best antibacterial activity in the ethylacetate fraction and ethanol 96% extract  at a concentration of 20% with the width of the zone of inhibition values of 4.375 mm and 4 mm.