Quantification of major royal jelly protein 1 in fresh royal jelly by indirect enzyme-linked immunosorbent assay

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Abstract

Rabbit polyclonal antibody produced by a major royal jelly protein 1 (MRJP1) specific peptide reacted only with a MRJP1. Indirect ELISA with the antibody revealed a MRJP1 level of 4.12-4.67 g/100 g in different company's royal jelly, which almost agreed with that of a hexametric form of MRJP1 (apisin) measured by high performance liquid chromatography. These results suggest that MRJP1 exists mainly as apisin in royal jelly.

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Yamaguchi, K., He, S., Li, Z., Murata, K., Hitomi, N., Mozumi, M., … Enomoto, T. (2013). Quantification of major royal jelly protein 1 in fresh royal jelly by indirect enzyme-linked immunosorbent assay. Bioscience, Biotechnology and Biochemistry, 77(6), 1310–1312. https://doi.org/10.1271/bbb.130013

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